Prep HPLC Column, Diamond Hydride, 4 um, 100 A. 21.2 mm ID x 250 mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 70000‑P21‑250 Diamond Hydride Preparative HPLC Column is designed for maximum‑resolution, application‑critical purification workflows where shorter preparative columns do not provide adequate separation. This full‑length preparative column delivers the highest resolving power within the 21.2 mm ID class while supporting large‑scale fraction collection.
Preparative Use Case: Why 21.2 mm ID × 250 mm
The 21.2 mm internal diameter defines this column as fully preparative, intended for laboratories performing milligram‑ to gram‑scale purification using dedicated preparative HPLC systems.
The 250 mm column length provides:
Maximum theoretical plate count in the preparative format
Superior resolution of closely eluting or structurally similar compounds
Higher peak capacity for highly complex polar mixtures
Increased confidence during high‑value fraction collection
This configuration is typically selected when resolution is the dominant requirement, and 50 mm, 100 mm, or 150 mm preparative columns cannot sufficiently resolve the target analytes.
Diamond Hydride™ TYPE‑C Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase delivers proven advantages for demanding preparative HILIC separations:
Highly stable and reproducible retention of polar analytes
Faster equilibration compared to conventional bonded‑silica HILIC phases
Excellent chemical stability under high‑organic mobile‑phase conditions
Long column lifetime in extended preparative use
This chemistry is especially effective for purification of:
Peptides and peptide‑like compounds
Metabolites and highly polar small molecules
Amine‑containing and basic analytes
Compounds best isolated using HILIC‑based retention mechanisms
Purpose‑Built for Maximum‑Resolution Preparative Work
The 70000‑P21‑250 enables predictable scale‑up from analytical and semi‑preparative Diamond Hydride methods while preserving chromatographic behavior. It is commonly used in method‑critical purification, advanced research, and regulated workflows where separation quality cannot be compromised.
System Requirements
Typical operating flow rates: ~20–30 mL/min
This column requires a preparative HPLC system capable of:
Sustained high‑flow solvent delivery
Large injection volumes
Pressure limits compatible with long preparative columns
Appropriate detector configurations and fraction collection
Standard analytical or semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase composition, viscosity, system pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
These columns represent a significant investment and are often application‑specific. To ensure customers receive the correct column for their workflow:
Pricing and technical support are provided through our Customer Service Department via formal quotation
Columns are produced on order, based on customer requirements
Orders cannot be canceled or returned once placed
Learn more about Custom Orders and our Custom Order policies.